ZWILCH Polyclonal Antibody

Applications

Tested Applications:

IHC, WB, ELISA

Species specificity:

human, mouse, rat

Positive WB detected in:

PC-3 cells

Positive IHC detected in:

human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:500-1:1000

IHC : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:

ZWILCH fusion protein Ag5454

Full name:

Zwilch, kinetochore associated, homolog (Drosophila)

Calculated molecular weight:

67 kDa

Observed molecular weight:

~60 kDa

Gene symbol

ZWILCH

Synonyms

hZwilch, KNTC1AP, Protein zwilch homolog, ZWILCH

Background

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.