CYP17A1 Rabbit pAb

Tested Applications

Applications:

WB,IHC-P,IHC-F,IF

Species specificity:

Mouse,Rat ( predicted:Human,Rabbit,Sheep,Cow,Horse )

Positive WB detected in:

HeLa cells, Jurkat cells, A549 cells, rat brain tissue, HepG2 cells, mouse brain tissue

Positive IHC detected in:

human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HepG2 cells

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:

CYP17A1 fusion protein Ag5899

Full name:

cytochrome P450, family 17, subfamily A, polypeptide 1

Calculated molecular weight:

57 kDa

Observed molecular weight:

48-51 kDa

Gene symbol

CYP17A1

Synonyms

CPT7, CYP17, CYP17A1, CYPXVII, Cytochrome P450 17A1, Cytochrome P450 C17, Cytochrome P450c17, P450C17, S17AH, Steroid 17 alpha monooxygenase

Background

The human cytochrome P450c17 enzyme is encoded by the CYP17A1 gene. It is the qualitative regulator of steroidogenesis in the human adrenals.In its absence, aldosterone is produced in the human adrenal zona glomerulosa .The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer