Casp1 | Caspase-1 p20 Rabbit pAb

Host: Rabbit 

Clonality: Polyclonal 

GeneID: 12362 

Isotype: IgG 

SWISS: P29452 

Applications: WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1μg/Test)

Target: Casp1 | Caspase-1 p20

Immunogen: KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402.

Reactivity: Human, Mouse, Rat

Purification: affinity purified by Protein A

Concentration: 1mg/ml

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Predicted MW.: 20/46 kDa

Observed MW.:45-47 kDa

Background: This gene encodes a protein which is a member of the cysteine- aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].

 Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20 at 1/300 dilution Secondary: IRDye800CW Goat Anti- Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD

 ample:lung (rat) Lysate at 40 ug Primary: Anti- Caspase-1 p20 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD

Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin- embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; 

Blocking buffer (normal goat serum) at 37∩ for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated 1:500, overnight at 4 ∑C, followed by conjugation to the secondary antibody and DAB staining